Unterschiede der Genexpression im Fettgewebe bei Kachexiepatienten mit Pankreaskarzinom

BACKGROUND: The loss of adipose tissue in cancer cachexia has been associated with different cytokines such as TNF-α, IL-1beta and IL-6, lipases PNPLA2 and LIPE, the protein ZAG and the protein MTOR.

OBJECTIVE: The aim of this study is to compare the RNA expression of the genes TNF-α, IL-1beta, LIPE, IL-6, PNPLA2, ZAG and MTOR in subcutaneous and visceral fat in patients with PDAC-induced cachexia to PDAC patients without cachexia. Additionally, the difference of the RNA expression in subcutaneous and visceral fat is analysed.

METHODS: RNA is isolated from subcutaneous and visceral fat tissue and then transcribed into cDNA. To measure levels of gene expressions, real-time quantitative PCR is performed.

RESULTS: The gene expression of TNF-α, IL-1beta, LIPE, IL-6, PNPLA2, ZAG and MTOR in patients with cachexia compared to the patients without cachexia was not significantly different in the analysed fat tissues. Moreover, the expression of the genes in subcutaneous fat compared to the expression in visceral fat showed no significant difference. ZAG expression in visceral fat was higher in cachectic patients compared to patients without cachexia. The difference was nearly significant.

CONCLUSION: This work confirms results of the literature on the increased ZAG expression in cachectic patients. Additional work is needed to confirm or reject hypotheses on the influence of cytokines, lipases and the protein MTOR in cachectic patients.